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KMID : 0376219780150010081
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Chonnam Medical Journal
1978 Volume.15 No. 1 p.81 ~ p.88
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Increased Synthesis of Ribose in Deoxygenated Erythrocytes
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Abstract
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Rabbit erythrocytes were incubated under nitrogen or oxygen atmosphere, and .the rates in the formation of ribose from glucose and in giycolysis(glucose consumption and lactate production) were determined.
When incubated under nitrogen, the erythrocytes showed rapid increases in the ribose formation as well as in glycolysis. However, lactate produced/glucose consumed(L; G) ratio was decreased in the nitrogen atmosphere, indicating an increase in the accumulation of glycolytic intermediates under anaerobic conditions. Inorganic phosphate added in the incubation media caused marked increases in the ribose synthesis and glycolysis in both atmospheres. The rise of the medium pH from 7.2 to 7.8 caused moderate increase in the ribose formation under nitrogen. In contrast, the oxygen atmosphere caused a slight decrease in the ribose formation at the alkaline pH in the presence of. inorganic phosphate. L/G ratios progressively decreased as the medium pH riled, but the decrement under nitrogen atmosphere was more prominent than under oxygen atmosphere. lodoacetate and fluoride, while inhibiting glycolysis, caused marked increases in the ribose level, whereas arsenate, which stimulated glycolysis, depleted the red cell endogenous ribose. In the nitrogen atmosphere, however, the effects of these metabolic effectors on the ribose formation were weakened to the similar extent as those on the glycolytic rate.
From these results, it is suggested that the enhanced synthesis of ribose in deoxygenated red cells results from increased accumulation of glycolytic intermediates which is primarily due to the intracellular pH change by deoxygenation of hemoglobin.
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